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| Carcinoma Cell Enrichment and Detection Kit - Immunocytochemistry |
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| Description |
The Carcinoma Cell Enrichment and Detection Kit – Immunocytochemistry is a detection system that allows a more than 10,000-fold enrichment and the subsequent immunocytochemical detection of disseminated epithelial tumor cells. The ready-to-use kit provides all necessary reagents for the magnetic enrichment and immunocytochemical detection of enriched tumor cells.
The carcinoma cells are permeabilized, fixed, and magnetically labeled with Anti-Cytokeratin MicroBeads. Magnetically labeled cells are then stained with a highly specific Anti-Cytokeratin-FITC antibody and Anti-FITC Alkaline Phosphatase and subjected to magnetic separation. After separation, cells are spun down onto a glass slide and stained with an alkaline phosphatase substrate. |
| Applications |
| The optimized enrichment and detection system allows the highly specific enrichment and subsequent detection of disseminated tumor cells in blood, bone marrow, or lymphoid tissue. |
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| Figure 1 |
| Immunocytochemical detection of breast cancer cells enriched using MACS® Technology and stained with Anti-Cytokeratin-FITC and Anti-FITC-Alkaline Phosphatase plus substrate. |
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| Products |
| Carcinoma Cell Enrichment and Detection Kit – Immunocytochemistry, human |
| For research use only |
- for 1-3×109 total cells
- for up to 20 tests
Components
- 2×50 mL 10× Dilution Buffer
- 50 mL MACS CellPerm Solution
- 50 mL MACS CellFix Solution
- 50 mL 10× MACS CellStain Solution
- 2 mL FcR Blocking Reagent
- 2 mL Anti-Cytokeratin MicroBeads
- 1 mL Anti-Cytokeratin (CK3-6H5)-FITC
- 100 μL Anti-FITC Alkaline Phosphatase
- 10 SIGMA FAST™ Fast Red TR/Naphthol AS-MX Alkaline Phosphatase Substrate Tablets
- 10 Tris/HCl tablets
Download datasheet
130-060-301
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| References |
| 1. Martin et al. (1998) Exp. Hematol. 26: 252–264. |
| 2. Gaforio et al. (2003) Int. J. Cancer 107: 984–990. |
| 3. Campos et al. (2008) J. Histochem. Cytochem. 56: 667–675. |
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