Annexin V Fluorochromes
Description The Annexin V Fluorochromes selectively label apoptotic, necrotic, and dead cells through the specific binding of Annexin V to phosphotidylserine (PS). In most normal, viable eukaryotic cells, the negatively charged phospholipid PS is located in the cytosolic leaflet of the plasma membrane lipid bilayer.1 PS redistribution from the inner to the outer leaflet is an early and widespread event during apoptosis.1,2 However, in necrosis, PS becomes accessible due to the disruption of membrane integrity.2 Apart from necrosis and apoptosis, PS also becomes accessible in activated platelets3, in certain cell anomalies like sickle cell anemia4, in erythrocyte senescence5, upon degranulation of mast cells6, and in certain stages of B cell differentiation7. PS exposure by apoptotic cells serves as a trigger for the recognition and removal by macrophages.8,9 Annexin V is a 35 kDa phospholipid-binding protein and has a high affinity for PS in the presence of physiological concentrations of calcium (Ca2+).10 Applications • Studies on cell death (apoptosis and/or necrosis). • Evaluation of MACS® Separations with the Annexin V MicroBead Kit		Figure 1 Staining procedure of Annexin V conjugates. Figure 2 Jurkat cells, cultured with staurosporine (50 nM) for 15 hours, were stained with Annexin V-FITC and analyzed by flow cytometry. Figure 3 Jurkat cells were cultured with and without different concentrations of staurosporine (200 nM up to 2 μM) for different times (from 4 up to 20.5 hours). Cells were then pooled and stained with Annexin V-Biotin followed by Anti-Biotin-APC and PI and analyzed by flow cytometry.

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