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| Mouse IgG1 - isotype control antibodies |
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| Description |
| The Mouse IgG1 isotype control antibody clone IS5-21F5 is specific for KLH (keyhole limpet hemocyanin). This protein is not expressed on human cells or cell lines. Therefore, the antibody clone IS5-21F5 can be used as a negative control, to distinguish specific from non-specific binding of mouse IgG1 fluorochrome-conjugated antibodies to human cells, for example via Fc receptors, or due to interactions of the fluorochrome with the cell surface. |
| Applications |
| Mouse IgG1 isotype control antibodies are suitable for assessing the level of background staining in flow cytometric analysis of human cells. |
| Clone | Isotype |
| IS5-21F5 | Mouse IgG1 |
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| Figure 1 |
| Human PBLs were stained with Mouse IgG1 antibodies conjugated to FITC (A), PE (B), APC (C), VioBlue (D), or PerCP (F), and analyzed by flow cytometry using the MACSQuant Analyzer. Cells stained with IgG1-Biotin (E) were stained with Anti-Biotin-APC in addition. |
| A |
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| B |
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| C |
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| D |
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| E |
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| F |
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| Products |
| Mouse IgG1-FITC |
- for 100 tests (1)
Download datasheet
130-092-213
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| Mouse IgG1-PE |
- for 100 tests (1)
Download datasheet
130-092-212
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| Mouse IgG1-APC |
- for 100 tests (1)
Download datasheet
130-092-214
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| Mouse IgG1-VioBlue |
- for 100 tests (1)
Download datasheet
130-094-670
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| Mouse IgG1-PerCP |
- for 100 tests (1)
Download datasheet
130-094-968
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| Mouse IgG1-Biotin |
- for 100 tests (2)
Download datasheet
130-093-018
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| (1) One test corresponds to fluorescent labling of up to 107 cells in a total volume of 100 µL. |
| (2) One test corresponds to labling of up to 107 cells in a total volume of 100µL. |
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