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| Description |
| Clone RA3-6B2 recognizes the mouse CD45R (B220) antigen which is expressed on B lymphocytes throughout their development from early pro-B stages onwards and is down-regulated upon terminal differentiation to plasma cells. Apart from B cells, CD45R is expressed on a small subset of dendritic cells (plasmacytoid dendritic cells). The CD45R monoclonal antibody clone RA3-6B2 specifically recognizes the exon A–restricted isoform of mouse CD45. CD45R is absent in thymus but reported to be present on apoptotic thymocytes. |
| Applications |
| The CD45R (B220) antibody can be used to identify and enumerate CD45R (B220)+ cells by flow cytometry or fluorescence microscopy. This antibody is suitable for MACS® Control applications. For further details, please refer to the data sheet of the separation reagent. |
| Clone | Isotype |
| RA3-6B2 | Rat IgG2a |
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| Figure 1 |
| Mouse splenocytes were stained with CD45R (B220) antibodies conjugated to FITC (A), PE (B), APC (C), VioBlue (D), or PerCP (E), and analyzed by flow cytometry using the MACSQuant® Analyzer. |
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| B |
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| C |
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| D |
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| E |
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| Products |
| CD45R (B220)-FITC, mouse |
- for 100 tests (1)
Download datasheet
130-091-829
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| CD45R (B220)-PE, mouse |
- for 100 tests (1)
Download datasheet
130-091-828
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| CD45R (B220)-APC, mouse |
- for 100 tests (1)
Download datasheet
130-091-843
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| CD45R (B220)-VioBlue, mouse |
- for 100 tests (1)
Download datasheet
130-094-287
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| CD45R (B220)-PerCP, mouse |
- for 100 tests (1)
Download datasheet
130-094-966
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| (1) One test corresponds to fluorescent labeling of up to 107 cells in a total volume of 100 μL. |
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