Human IL-4
Description Human interleukin 4 (IL-4) is a pleiotropic cytokine, which plays a central role in humoral and adaptive immune responses. IL-4 is predominantly secreted by activated CD4+ memory and effector TH2 cells, basophils, and mast cells. It promotes the proliferation and differentiation of B cells, as well as immunoglobulin isotype switching, and MHC class II antigen and low-affinity IgE receptor expression. Furthermore, IL-4 induces the differentiation of naive CD4+ T cells into helper TH2 cells, while suppressing TH1 development, and promotes chemotaxis of mast cells and basophils. Excessive IL-4 production and mechanisms involving TH2 types have been associated with immunological disorders, such as IgE-mediated allergy. Applications Human IL-4 can be used for a variety of applications including: in vitro generation of Mo-DCs (e.g. together with GM-CSF)1, in vitro differentiation of naive CD4+ T cells towards TH2 cells Quality description Research grade Research grade is the standard quality for all our products. They are sterile-filtered and have passed an accurate quality control. Generally, endotoxin levels are below 0.1 ng/µg (< 1 EU/µg), and purities are higher than 95%. The biological activity has usually been tested employing appropriate bioassays, and a minimum activity is assured. Otherwise the ED50 value is given. Premium grade For demanding applications, selected human cytokines and growth factors are available in premium grade quality having a particular high and well-defined biological activity. They have passed stringent quality control criteria, and the purity is higher than 97%. The biological activity has been determined after lyophilization in direct comparison with the respective WHO standards. Certificates of analysis stating lot-specific biological activities are available on request. Furthermore, premium grade cytokines display the major characteristics of our line of GMP grade cytokines. Premium grade products are widely used, e.g, for the generation of dendritic cells and the cultivation of stem cells. Cell culture grade Cell culture grade cytokines and growth factors are lyophilized with DMEM/F12 and fetal bovine serum or human serum albumin. These products are well-suited for certain applications where the direct reconstitution with water is favorable, e.g., applications with small volumes and tests in 96-well plates.		Figure 1 Human IL-4 activity assay. The biological activity of Human IL-4, premium grade was determined by proliferation assay using TF-1 cells.

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